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Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement.
| Content Provider | Europe PMC |
|---|---|
| Author | Bazsefidpar, Shayesteh Serrano-Pertierra, Esther Gutiérrez, Gemma Calvo, Alberto Sánchez Matos, María Blanco-López, María Carmen |
| Abstract | AbstractThe aim of this study was to develop a sensitive lateral flow immunoassay (LFIA) for the rapid detection of Escherichia coli (E. coli) O157:H7, a pathogen contributor to diseases and fatalities worldwide. Au nanoparticles with high stability, uniform size, and shape were synthesized and coated with heterobifunctional PEG polymer with carboxyl groups, and they were bioconjugated to be used as label in sandwich-LFIA. Then, a silver enhancement strategy was developed as an accessible, rapid, and cost-effective approach for signal amplification to reduce the limit of detection (LOD). The optimal results were achieved when a solution of silver nitrate and hydroquinone/citrate buffer was added to the strips for 4 min. This led to a decrease in the visual LOD from 2 × 106 (CFU mL−1) to 2 × 103 (CFU mL−1), resulting in a threefold improvement in sensitivity compared to the conventional LFIA system. The specificity of the system was evaluated by using non-target bacteria (E. coli BL21 and E. coli T515) and its reliability was determined by testing commercial food samples (milk, tap water, and orange juice), demonstrating its effectiveness for quickly detecting pathogenic bacteria in food products.Graphical AbstractSupplementary InformationThe online version contains supplementary material available at 10.1007/s00604-023-05834-8. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10279566&blobtype=pdf |
| ISSN | 00263672 |
| Journal | Mikrochimica Acta [Mikrochim Acta] |
| Volume Number | 190 |
| DOI | 10.1007/s00604-023-05834-8 |
| PubMed Central reference number | PMC10279566 |
| Issue Number | 7 |
| PubMed reference number | 37336818 |
| e-ISSN | 14365073 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2023-06-19 |
| Publisher Place | Vienna |
| Access Restriction | Open |
| Rights License | Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2023 |
| Subject Keyword | E. coli O157:H7 Silver enhancement PEG polymer Sandwich-LFIA Food analysis Foodborne pathogens |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry |
