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Development and Validation of a New LC-MS/MS Bioanalytical Method for the Simultaneous Determination of Levodopa, Levodopa Methyl Ester, and Carbidopa in Human Plasma Samples.
| Content Provider | Europe PMC |
|---|---|
| Author | Molteni, Linda Charlier, Bruno Izzo, Viviana Coglianese, Albino Conti, Valeria Eleopra, Roberto Cilia, Roberto Capelli, Chiara D’Urso, Annachiara de Grazia, Ugo |
| Editor | Tsakalof, Andreas Zacharis, Constantinos K. |
| Copyright Year | 2023 |
| Abstract | Levodopa (L-DOPA) treatment, combined with the administration of dopa-decarboxylase inhibitors (DDCIs), is still the most effective symptomatic treatment of Parkinson's disease (PD). Although its efficacy in the early stage of the disease has been confirmed, its complex pharmacokinetics (PK) increases the variability of the intra-individual motor response, thus amplifying the risk of motor/non-motor fluctuations and dyskinesia. Moreover, it has been demonstrated that L-DOPA PK is strongly influenced by several clinical, therapeutic, and lifestyle variables (e.g., dietary proteins). L-DOPA therapeutic monitoring is therefore crucial to provide personalized therapy, hence improving drug efficacy and safety. To this aim, we have developed and validated an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to quantify L-DOPA, levodopa methyl ester (LDME), and the DDCI carbidopa in human plasma. The compounds were extracted by protein precipitation and samples were analyzed with a triple quadrupole mass spectrometer. The method showed good selectivity and specificity for all compounds. No carryover was observed, and dilution integrity was demonstrated. No matrix effect could be retrieved; intra-day and inter-day precision and accuracy values met the acceptance criteria. Reinjection reproducibility was assessed. The described method was successfully applied to a 45-year-old male patient to compare the pharmacokinetic behavior of an L-DOPA-based medical treatment involving commercially available Mucuna pruriens extracts and an LDME/carbidopa (100/25 mg) formulation. |
| Journal | Molecules |
| Volume Number | 28 |
| DOI | 10.3390/molecules28114264 |
| PubMed Central reference number | PMC10254897 |
| Issue Number | 11 |
| PubMed reference number | 37298741 |
| e-ISSN | 14203049 |
| Language | English |
| Publisher | Molecular Diversity Preservation International (MDPI) |
| Publisher Date | 2023-05-23 |
| Access Restriction | Open |
| Subject Keyword | levodopa levodopa methyl ester carbidopa Parkinson’s disease therapeutic drug monitoring liquid chromatography–mass spectrometry (LC–MS) analytical method |
| Content Type | Text |
| Resource Type | Case study |
| Subject | Physical and Theoretical Chemistry Medicine Chemistry Drug Discovery Pharmaceutical Science Analytical Chemistry Molecular Medicine Organic Chemistry |
