UHPLC-QTOF-MS Metabolic Profiling of Marchantia polymorpha and Evaluation of Its Hepatoprotective Activity Using Paracetamol-Induced Liver Injury in Mice.

Content Provider	Europe PMC
Author	Asif, Ayesha Ishtiaq, Saiqa Kamran, Sairah Hafeez Youssef, Fadia S. Lashkar, Manar O. Ahmed, Safwat A. Ashour, Mohamed L.
Copyright Year	2023
Abstract	Marchantia species weretraditionallyused to treat liver failure. Marchantia polymorpha chloroform extract showed a marked hepatoprotective activity ina dose-dependent manner in paracetamol-induced extensive liver damagein mice. At a dose of 500 mg/kg (MP-500), it resulted in a reductionin aspartate transaminase by 49.44%, alanine transaminase by 44.11%,and alkaline phosphatase by 24.4% with significant elevation in totalproteins by 58.69% with respect to the diseased group. It showed significantreductions in total bilirubin, total cholesterol, triglycerides, lowdensity lipoprotein (LDL), very LDL, total lipids, and to high densitylipoprotein ratio (CH/HDL) by 53.42, 30.14, 35.02, 45.79, 34.74, 41.45,and 49.52%, respectively, together with a 37.69% increase in HDL withrespect to the diseased group. It also showed an elevation of superoxidedismutase by 28.09% and in glutathione peroxidase by 81.83% in additionto the reduction of lipid peroxidation by 17.95% as compared to theparacetamol only treated group. This was further supported by histopathologicalexamination that showed normal liver architecture and a normal sinusoidalgap. Metabolic profiling by ultrahigh performance liquid chromatographycoupled with quadrupole time-of-flight mass spectrometer (UHPLC–QTOF/MS)led to the tentative identification of 28 compounds belonging to phenols,quinolones, phenylpropanoid, acylaminosugars, terpenoids, lipids,and fatty acids to which the activity was attributed. Four compoundswere detected in the negative ionization mode which are neoacrimarineJ, marchantin A, chitobiose, and phellodensin F, while the rest weredetected in the positive mode. Thus, it can be concluded that thisplant could serve as a valuable choice for the treatment of hepatotoxicitythat further consolidated its traditional use.
Journal	ACS Omega
Volume Number	8
PubMed Central reference number	PMC10233839
Issue Number	21
PubMed reference number	37273612
e-ISSN	24701343
DOI	10.1021/acsomega.3c01867
Language	English
Publisher	American Chemical Society
Publisher Date	2023-05-17
Access Restriction	Open
Rights License	Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). © 2023 The Authors. Published by American Chemical Society
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering