Localized Surface Plasmon Resonance-Based Nanosensor for Rapid Detection of Glyphosate in Food Samples.

Content Provider	Europe PMC
Author	Côco, Ariany Soares Campos, Fabiana Vasconcelos Díaz, Camilo Arturo Rodríguez Guimarães, Marco César Cunegundes Prado, Adilson Ribeiro de Oliveira, Jairo Pinto
Copyright Year	2023
Abstract	In this study, we developed a biosensor based on the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles (AuNPs) to detect the widely used herbicide glyphosate in food samples. To do so, either cysteamine or a specific antibody for glyphosate were conjugated to the surface of the nanoparticles. AuNPs were synthesized using the sodium citrate reduction method and had their concentration determined via inductively plasma coupled mass spectrometry. Their optical properties were analyzed using UV-vis spectroscopy, X-ray diffraction, and transmission electron microscopy. Functionalized AuNPs were further characterized via Fourier-transform infrared spectroscopy, Raman scattering, Zeta potential, and dynamic light scattering. Both conjugates succeeded in detecting the presence of glyphosate in the colloid, although nanoparticles functionalized with cysteamine tended to aggregate at high concentrations of the herbicide. On the other hand, AuNPs functionalized with anti-glyphosate functioned at a broad concentration range and successfully identified the presence of the herbicide in non-organic coffee samples and when it was added to an organic coffee sample. This study demonstrates the potential of AuNP-based biosensors to detect glyphosate in food samples. The low-cost and specificity of these biosensors make them a viable alternative to current methods for detecting glyphosate in foodstuffs.
Volume Number	13
PubMed Central reference number	PMC10216528
Issue Number	5
PubMed reference number	37232873
Journal	Biosensors (Basel)
e-ISSN	20796374
DOI	10.3390/bios13050512
Language	English
Publisher	MDPI
Publisher Date	2023-04-30
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	biosensing glyphosate gold nanoparticles localized surface plasmon resonance cysteamine anti-glyphosate antibody
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology