Agarose gel electrophoresis pattern of serum creatine kinase and lactate dehydrogenase isoenzymes in zoo-managed Asian elephants (Elephas maximus).

Content Provider	Europe PMC
Author	TAKEHANA, Kazuya ADACHI, Mare ISHIKAWA, Shingo YAMAGISHI, Norio
Copyright Year	2023
Abstract	Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) isoenzymes were evaluated in nine zoo-managed Asian elephants (Elephas maximus) using a commercialagarose gel electrophoresis (AGE) kit. CK was separated into two major fractions, CK-BB and CK-MM, along with a small fraction of macroenzyme-CK type 2 (mCK2); CK-MM was the largestfraction. LDH was separated into five fractions (LDH1–5); LDH3 was the largest fraction. Age was negatively and positively correlated with the percentages of CK-BB and CK-MM, respectively,and negatively correlated with CK-BB and mCK2 activities. These results indicate that an AGE kit can be used to evaluate CK and LDH isoenzymes. Routine isoenzyme testing may enable earlydetection of disease and physiological changes.
ISSN	09167250
Volume Number	85
PubMed Central reference number	PMC10209465
Issue Number	5
PubMed reference number	36927856
Journal	The Journal of Veterinary Medical Science [J. Vet. Med. Sci]
e-ISSN	13477439
DOI	10.1292/jvms.22-0550
Language	English
Publisher	The Japanese Society of Veterinary Science
Publisher Date	2023-03-14
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) ©2023 The Japanese Society of Veterinary Science
Subject Keyword	agarose gel electrophoresis (AGE) Asian elephants (Elephas maximus) creatine kinase (CK) isoenzyme lactate dehydrogenase (LDH)
Content Type	Text
Resource Type	Article
Subject	Veterinary