Amino acid profile changes during enrichment of spheroid cells with cancer stem cell properties in MCF-7 and MDA-MB-231 cell lines.

Content Provider	Europe PMC
Author	Ghanbari Movahed, Zahra Matin, Maryam M. Mansouri, Kamran Sisakhtnezhad, Sajjad
Abstract	AbstractBackgroundCancer stem cells (CSCs), subpopulations of cancer cells, are responsible for tumor progression, metastasis, and relapse. Changes in amino acid metabolism are linked to breast cancer recurrence and metastasis.AimsThis study aimed to evaluate the changes in the amino acid profile in MCF‐7 and MDA‐MB‐231 cells during spheroid formation to discover the specific metabolic properties in CSCs.MethodsMCF‐7 and MDA‐MB‐231 breast cancer cells were cultured as spheroids and evaluated to characterize their CSC properties. The characteristics of CSC were evaluated by examining the expression of CSC markers and conducting drug resistance assays. In addition, amino acid profile change during the enrichment of breast cancer stem cells in the spheroids was investigated by high‐performance liquid chromatography (HPLC).ResultsThe results indicated that out of 20 different amino acids analyzed, 19 of them decreased during the spheroid formation process. Alanine, lysine, phenylalanine, threonine, and glycine showed significant reductions in the conditioned media of both cell lines in the spheroid form compared to the monolayer cells. Only one of the amino acids increased in MCF‐7 and MDA‐MB‐231 spheroids (histidine and serine, respectively).ConclusionOur results suggest that certain amino acids identified in this study can be used for a better understanding of the molecular mechanisms associated with breast cancer stem cell formation.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10172158&blobtype=pdf
Page Count	9
Journal	Cancer Reports [Cancer Rep (Hoboken)]
Volume Number	6
DOI	10.1002/cnr2.1809
PubMed Central reference number	PMC10172158
Issue Number	5
PubMed reference number	37092500
e-ISSN	25738348
Language	English
Publisher	John Wiley and Sons Inc.
Publisher Date	2023-04-24
Publisher Place	Hoboken
Access Restriction	Open
Rights License	This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2023 The Authors. Cancer Reports published by Wiley Periodicals LLC.
Subject Keyword	amino acid metabolism breast cancer cancer stem cells MCF‐7 MDA‐MB‐231 spheroid culture
Content Type	Text
Resource Type	Article
Subject	Oncology Cancer Research