Gene expression patterns and related pathways in the hearts of rhesus monkeys subjected to prolonged myocardial ischemia.

Content Provider	Europe PMC
Author	Liu, Xiaojuan Zhang, Jingyao Li, Pengfei Han, Pengfei Kang, Y James Zhang, Wenjing
Copyright Year	2023
Description	After myocardial infarction (MI) occurs, progressive pathological cardiac remodeling results in heart dysfunction and even heart failure during the following months or years. The present study explored the molecular mechanisms underlying the late phase of MI at the global transcript level. A rhesus monkey model of myocardial ischemia induced by left anterior descending (LAD) artery ligation was established, and the heart tissue was collected eight weeks after ligation for transcriptome analysis by DNA microarray technology. Differentially expressed genes in the core infarcted area and remote infarcted area of the ischemic heart were detected with significance analysis of microarray (SAM), and related pathways were detected by Gene Ontology (GO)/pathway analysis. We found that compared to the sham condition, prolonged ischemia increased the levels of 941 transcripts, decreased the levels of 380 transcripts in the core infarcted area, and decreased the levels of 8 transcripts in the remote area in monkey heart tissue. Loss of coordination between the expression of genes, including natriuretic peptide A (NPPA), NPPB, and corin (Corin, serine peptidase), may aggravate cardiac remodeling. Furthermore, imbalance in the enriched significantly changed pathways, including fibrosis-related pathways, cardioprotective pathways, and the cardiac systolic pathway, likely also plays a key role in regulating the development of heart remodeling.
Abstract	After myocardial infarction (MI) occurs, progressive pathological cardiacremodeling results in heart dysfunction and even heart failure during thefollowing months or years. The present study explored the molecular mechanismsunderlying the late phase of MI at the global transcript level. A rhesus monkeymodel of myocardial ischemia induced by left anterior descending (LAD) arteryligation was established, and the heart tissue was collected eight weeks afterligation for transcriptome analysis by DNA microarray technology. Differentiallyexpressed genes in the core infarcted area and remote infarcted area of theischemic heart were detected with significance analysis of microarray (SAM), andrelated pathways were detected by Gene Ontology (GO)/pathway analysis. We foundthat compared to the sham condition, prolonged ischemia increased the levels of941 transcripts, decreased the levels of 380 transcripts in the core infarctedarea, and decreased the levels of 8 transcripts in the remote area in monkeyheart tissue. Loss of coordination between the expression of genes, includingnatriuretic peptide A (NPPA), NPPB, andcorin (Corin, serine peptidase), may aggravate cardiacremodeling. Furthermore, imbalance in the enriched significantly changedpathways, including fibrosis-related pathways, cardioprotective pathways, andthe cardiac systolic pathway, likely also plays a key role in regulating thedevelopment of heart remodeling.
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ISSN	15353702
Volume Number	248
DOI	10.1177/15353702231151968
PubMed Central reference number	PMC10159524
Issue Number	4
PubMed reference number	36814407
Journal	Experimental Biology and Medicine [Exp Biol Med (Maywood)]
e-ISSN	15353699
Language	English
Publisher	SAGE Publications
Publisher Date	2023-02-22
Publisher Place	Sage UK: London, England
Access Restriction	Open
Rights License	© 2023 by the Society for Experimental Biology andMedicine
Subject Keyword	Myocardial infarction prolonged ischemia DNA microarray technology gene expression pathways rhesus monkey
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology