Green Extracts and UPLC-TQS-MS/MS Profiling of Flavonoids from Mexican Oregano (Lippia graveolens) Using Natural Deep Eutectic Solvents/Ultrasound-Assisted and Supercritical Fluids.

Content Provider	Europe PMC
Author	Bernal-Millán, Manuel de Jesús Carrasco-Portugal, Miriam del Carmen Heredia, J. Basilio Bastidas-Bastidas, Pedro de Jesús Gutiérrez-Grijalva, Erick Paul León-Félix, Josefina Angulo-Escalante, Miguel Ángel
Editor	Kreft, Ivan
Copyright Year	2023
Abstract	Mexican oregano (Lippia graveolens) is an important source of bioactive compounds, such as flavonoids. These have presented different therapeutic properties, including antioxidant and anti-inflammatory; however, their functionality is related to the quantity and type of compounds, and these characteristics depend on the extraction method used. This study aimed to compare different extraction procedures to identify and quantify flavonoids from oregano (Lippia graveolens). Emerging and conventional technologies include maceration with methanol and water, and ultrasound-assisted extraction (UAE) using deep eutectic solvents (DES) such as choline chloride-ethylene glycol, choline chloride-glycerol, and choline chloride-lactic acid. Supercritical fluid extraction using CO2 as a solvent was also studied. Six different extracts were obtained and the total reducing capacity, total flavonoid content, and antioxidant capacity by ABTS•+, DPPH•, FRAP, and ORAC were evaluated. In addition, flavonoids were identified and quantified by UPLC-TQS-MS/MS. Results showed that UAE-DES had the best extraction effect and antioxidant capacity using colorimetric methods. However, maceration-methanol was superior in compound content, and highlighting naringenin and phloridzin were the major compounds. In addition, this extract was microencapsulated by spray drying, which provided a protection feature of their antioxidant potential. Oregano extracts are rich in flavonoids and the microcapsules present promising results for future research.
Journal	Plants (Basel, Switzerland)
Volume Number	12
PubMed Central reference number	PMC10145289
Issue Number	8
PubMed reference number	37111915
e-ISSN	22237747
DOI	10.3390/plants12081692
Language	English
Publisher	MDPI
Publisher Date	2023-04-18
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	flavonoids oregano antioxidant capacity extraction techniques microcapsules chromatographic profile
Content Type	Text
Resource Type	Article
Subject	Plant Science Ecology Ecology, Evolution, Behavior and Systematics