NDLI: Effect of Extraction Time on the Extractability and Physicochemical Properties of Pepsin-Soluble Collagen (PCS) from the Skin of Silver Catfish (<i>Pangasius</i> sp.).

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Effect of Extraction Time on the Extractability and Physicochemical Properties of Pepsin-Soluble Collagen (PCS) from the Skin of Silver Catfish (Pangasius sp.).

Content Provider	Europe PMC
Author	Shaik, Mannur Ismail Md Nor, Intan Nordiana Sarbon, Norizah Mhd
Editor	Giannakas, Aris
Copyright Year	2023
Abstract	The current study aimed to determine the effects of extraction time on the extractability and physicochemical properties of collagen from the skin of silver catfish (Pangasius sp.). Pepsin soluble collagen (PSC) was extracted for 24 and 48 h and analysed in terms of chemical composition, solubility, functional group, microstructure, and rheological properties. The yields of PSC at 24 h and 48 h extraction time were 23.64% and 26.43%, respectively. The chemical composition exhibited significant differences, with PSC extracted at 24 h showing better moisture, protein, fat, and ash content. Both collagen extractions indicated the highest solubility at pH 5. In addition, both collagen extractions exhibited Amide A, I, II, and III as fingerprint regions for collagen structure. The morphology of the extracted collagen appeared porous with a fibril structure. The dynamic viscoelastic measurements of complex viscosity (η*) and loss tangent (tan δ) decreased as temperature increased, and the viscosity increased exponentially as the frequency increased, whereas the loss tangent decreased. In conclusion, PSC extracted at 24 h showed similar extractability to that extracted at 48 h but with a better chemical composition and shorter extraction time. Therefore, 24 h is the best extraction time for PSC from silver catfish skin.
Journal	Gels
Volume Number	9
PubMed Central reference number	PMC10137522
Issue Number	4
PubMed reference number	37102912
e-ISSN	23102861
DOI	10.3390/gels9040300
Language	English
Publisher	MDPI
Publisher Date	2023-04-03
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	collagen yield extraction time physicochemical properties silver catfish
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomaterials Polymers and Plastics Organic Chemistry

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