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The Employment of the Surface Plasmon Resonance (SPR) Microscopy Sensor for the Detection of Individual Extracellular Vesicles and Non-Biological Nanoparticles.
| Content Provider | Europe PMC |
|---|---|
| Author | Sharar, Nour Wüstefeld, Konstantin Talukder, Rahat Morad Skolnik, Julija Kaufmann, Katharina Giebel, Bernd Börger, Verena Nolte, Friedrich Watzl, Carsten Weichert, Frank Hergenröder, Roland Shpacovitch, Victoria |
| Copyright Year | 2023 |
| Abstract | A wide-field surface plasmon resonance (SPR) microscopy sensor employs the surface plasmon resonance phenomenon to detect individual biological and non-biological nanoparticles. This sensor enables the detection, sizing, and quantification of biological nanoparticles (bioNPs), such as extracellular vesicles (EVs), viruses, and virus-like particles. The selectivity of bioNP detection does not require biological particle labeling, and it is achieved via the functionalization of the gold sensor surface by target-bioNP-specific antibodies. In the current work, we demonstrate the ability of SPR microscopy sensors to detect, simultaneously, silica NPs that differ by four times in size. Employed silica particles are close in their refractive index to bioNPs. The literature reports the ability of SPR microscopy sensors to detect the binding of lymphocytes (around 10 μm objects) to the sensor surface. Taken together, our findings and the results reported in the literature indicate the power of SPR microscopy sensors to detect bioNPs that differ by at least two orders in size. Modifications of the optical sensor scheme, such as mounting a concave lens, help to achieve homogeneous illumination of a gold sensor chip surface. In the current work, we also characterize the improved magnification factor of the modified SPR instrument. We evaluate the effectiveness of the modified and the primary version of the SPR microscopy sensors in detecting EVs isolated via different approaches. In addition, we demonstrate the possibility of employing translation and rotation stepper motors for precise adjustments of the positions of sensor optical elements—prism and objective—in the primary version of the SPR microscopy sensor instrument, and we present an algorithm to establish effective sensor–actuator coupling. |
| Volume Number | 13 |
| PubMed Central reference number | PMC10136938 |
| Issue Number | 4 |
| PubMed reference number | 37185547 |
| Journal | Biosensors (Basel) |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios13040472 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2023-04-12 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors. |
| Subject Keyword | surface plasmon resonance (SPR) SPR microscopy extracellular vesicles viruses virus-like particles sensor imaging deep learning imaging artifacts sensor–actuator coupling |
| Content Type | Text |
| Resource Type | Article |
| Subject | Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology |
