Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells.

Content Provider	Europe PMC
Author	Du, Wei Gong, Xiu-Lin Tian, Yang Zhu, Xi Peng, Yu Wang, Ya-Wen
Copyright Year	2023
Abstract	In this work, a coumarin derivative, SWJT-14, was synthesized as a fluorescence probe to distinguish cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions. The detection limit of Cys, Hcy and GSH for the probe was 0.02 μM, 0.42 μM and 0.92 μM, respectively, which was lower than biothiols in cells. The probe reacted with biothiols to generate different products with different conjugated structures. Additionally, it could distinguish Cys, Hcy and GSH using fluorescence and UV-Vis spectra. The detection mechanism was confirmed by MS. SWJT-14 was successfully used in cellular experiments and detected both endogenous and exogenous biothiols.
Volume Number	13
PubMed Central reference number	PMC10136212
Issue Number	4
PubMed reference number	37185522
Journal	Biosensors (Basel)
e-ISSN	20796374
DOI	10.3390/bios13040447
Language	English
Publisher	MDPI
Publisher Date	2023-03-31
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	biothiols fluorescence coumarin derivative
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology