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Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties.
| Content Provider | Europe PMC |
|---|---|
| Author | Tiangpook, Suratsawadee Nhim, Sreyneang Prangthip, Pattaneeya Pason, Patthra Tachaapaikoon, Chakrit Ratanakhanokchai, Khanok Waeonukul, Rattiya |
| Editor | Krastanov, Albert Ivanov |
| Copyright Year | 2023 |
| Abstract | Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without contabacillusmination from glucose and maltose was achieved at 36 h of cultivation using 50 g/L of maltose, with a yield of 72.7%. IMOs were purified by size exclusion chromatography with a Superdex 30 Increase column. The molecular mass and DP of IMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Subsequently, linkages in produced oligosaccharides were verified by enzymatic hydrolysis with α-amylase and oligo-α-1,6-glucosidase. These IMOs showed prebiotic properties, namely tolerance to acidic conditions and digestive enzymes of the gastrointestinal tract, stimulation of probiotic bacteria growth to produce short-chain fatty acids and no stimulating effect on pathogenic bacteria growth. Moreover, these IMOs were not toxic to mammalian cells at up to 5 mg/mL, indicating their biocompatibility. Therefore, this research demonstrated a simple and economical method for producing IMOs with DP2–14 without additional operations; moreover, the excellent prebiotic properties of the IMOs offer great prospects for their application in functional foods. |
| Journal | Foods (Basel, Switzerland) |
| Volume Number | 12 |
| PubMed Central reference number | PMC10094464 |
| Issue Number | 7 |
| PubMed reference number | 37048320 |
| e-ISSN | 23048158 |
| DOI | 10.3390/foods12071499 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2023-04-03 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors. |
| Subject Keyword | Bacillus subtilis α-glucosidase isomaltooligosaccharide prebiotic transglucosidase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Health (social science) Health Professions Food Science Plant Science |
