Simultaneous Determination of 26 Pesticide Residues in Traditional Chinese Medicinal Leeches by Modified QuEChERS Coupled with HPLC-MS/MS.

Content Provider	Europe PMC
Author	Zhou, Ying Yu, Yahui Huang, Qian Zheng, Huixin Zhan, Ruyi Chen, Luting Meng, Xingang
Copyright Year	2023
Abstract	A Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS)pretreatmenttechnique combined with HPLC–MS/MS was established to detect26 pesticides in traditional Chinese medicinal leeches. The samplewas extracted by acetonitrile solution with sodium acetate-0.1% (v/v)acetic acid as a buffer system, then cleaned up by a mixture of 750mg of MgSO4, 150 mg of C18, and 150 mg of PSA,separated by an ACQUITY BEH C18 column, and determinedin the dynamic multiple reaction mode. Under the optimized conditions,the peak areas of the 26 pesticides in leeches showed good linearity(r > 0.99) between their mass concentrations from1 to 100 μg/L. At the spike levels of 10, 20, and 100 μg/kg,the recoveries of 26 pesticides in leeches were 72.9–101.6%with an RSD of 1.1–12.8%, an LOQ of 10 μg/kg, and anLOD of 0.1–5.4 μg/kg. This method is easy, rapid, sensitive,and practical and meets the requirements of pesticide residue detectionstandards.
Journal	ACS Omega
Volume Number	8
PubMed Central reference number	PMC10077569
Issue Number	13
PubMed reference number	37033865
e-ISSN	24701343
DOI	10.1021/acsomega.3c00217
Language	English
Publisher	American Chemical Society
Publisher Date	2023-03-22
Access Restriction	Open
Rights License	Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). © 2023 The Authors. Published by American Chemical Society
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering