TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model.

Content Provider	Europe PMC
Author	Sciurti, Elisa Blasi, Laura Prontera, Carmela Tania Barca, Amilcare Giampetruzzi, Lucia Verri, Tiziano Siciliano, Pietro Aleardo Francioso, Luca
Editor	David, Iulia Gabriela Buleandra, Mihaela
Copyright Year	2023
Abstract	Monitoring of ions in real-time directly in cell culture systems and in organ-on-a-chip platforms represents a significant investigation tool to understand ion regulation and distribution in the body and ions’ involvement in biological mechanisms and specific pathologies. Innovative flexible sensors coupling electrochemical stripping analysis (square wave anodic stripping voltammetry, SWASV) with an ion selective membrane (ISM) were developed and integrated in Transwell™ cell culture systems to investigate the transport of zinc and copper ions across a human intestinal Caco-2 cell monolayer. The fabricated ion-selective sensors demonstrated good sensitivity (1 × 10−11 M ion concentration) and low detection limits, consistent with pathophysiological cellular concentration ranges. A non-invasive electrochemical impedance spectroscopy (EIS) analysis, in situ, across a selected spectrum of frequencies (10–105 Hz), and an equivalent circuit fitting were employed to obtain useful electrical parameters for cellular barrier integrity monitoring. Transepithelial electrical resistance (TEER) data and immunofluorescent images were used to validate the intestinal epithelial integrity and the permeability enhancer effect of ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) treatment. The proposed devices represent a real prospective tool for monitoring cellular and molecular events and for studies on gut metabolism/permeability. They will enable a rapid integration of these sensors into gut-on-chip systems.
Journal	Micromachines (Basel)
Volume Number	14
PubMed Central reference number	PMC10054836
Issue Number	3
PubMed reference number	36984903
e-ISSN	2072666X
DOI	10.3390/mi14030496
Language	English
Publisher	MDPI
Publisher Date	2023-02-21
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	organ-on-a-chip (OoC) anodic stripping voltammetry transepithelial electrical resistance (TEER) electrochemical impedance spectroscopy (EIS) Caco-2 monolayer Transwell™ plate
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering Control and Systems Engineering Mechanical Engineering