NDLI: Antibacterial Activity of Epigallocatechin Gallate (EGCG) against <i>Shigella flexneri</i>.

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Antibacterial Activity of Epigallocatechin Gallate (EGCG) against Shigella flexneri.

Content Provider	Europe PMC
Author	Zhang, Yini Zhang, Yeyue Ma, Ruiqing Sun, Wanting Ji, Zheng
Editor	De Francesco, Maria Antonia
Copyright Year	2023
Abstract	Shigella flexneri (S. flexneri), a major intestinal pathogen, is a global public health concern. The biofilms formed by S. flexneri threaten environmental safety, since they could promote the danger of environmental contamination and strengthen the disease-causing properties of bacteria. Epigallocatechin gallate (EGCG) is an important catechin in tea, which has a high antibacterial activity. However, its antibacterial mechanism is still unclear. This research aims to quantify the antibacterial function and investigate the possible mechanism of EGCG inhibition of S. flexneri. The minimum inhibitory concentration (MIC) of EGCG against planktonic S. flexneri in the investigation was measured to be 400 μg/mL. Besides, SDS-PAGE and field emission scanning electron microscopy showed that EGCG interfered with protein synthesis and changed bacteria morphology. Through controlling the expression of the mdoH gene, EGCG was found to be able to prevent an S. flexneri biofilm extracellular polysaccharide from forming, according to experiments utilizing the real-time PCR test. Additional research revealed that EGCG might stimulate the response of S. flexneri to oxidative stress and prevent bacterial growth. These findings suggest that EGCG, a natural compound, may play a substantial role in S. flexneri growth inhibition.
ISSN	16617827
Journal	International Journal of Environmental Research and Public Health [Int J Environ Res Public Health]
Volume Number	20
DOI	10.3390/ijerph20064676
PubMed Central reference number	PMC10048926
Issue Number	6
PubMed reference number	36981585
e-ISSN	16604601
Language	English
Publisher	Molecular Diversity Preservation International (MDPI)
Publisher Date	2023-03-07
Access Restriction	Open
Subject Keyword	epigallocatechin gallate biofilm Shigella flexneri inhibition mechanism oxidative stress
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Pollution Public Health, Environmental and Occupational Health

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