Genome-wide identification and expression analysis of diacylglycerol acyltransferase genes in soybean (Glycine max).

Content Provider	Europe PMC
Author	Zhao, Shihui Yan, Fan Liu, Yajing Sun, Monan Wang, Ying Li, Jingwen Zhang, Xinsheng Yang, Xuguang Wang, Qingyu
Editor	Uversky, Vladimir
Copyright Year	2023
Abstract	BackgroundSoybean (Glycine max) is a major protein and vegetable oil source. In plants, diacylglycerol acyltransferase (DGAT) can exert strong flux control, which is rate-limiting for triacylglycerol biosynthesis in seed oil formation.MethodsHere, we identified soybean DGAT genes via a bioinformatics method, thereby laying a solid foundation for further research on their function. Based on our bioinformatics analyses, including gene structure, protein domain characteristics, and phylogenetic analysis, 26 DGAT putative gene family members unevenly distributed on 12 of the 20 soybean chromosomes were identified and divided into the following four groups: DGAT1, DGAT2, WS/DGAT, and cytoplasmic DGAT.ResultsThe Ka/Ks ratio of most of these genes indicated a significant positive selection pressure. DGAT genes exhibited characteristic expression patterns in soybean tissues. The differences in the structure and expression of soybean DGAT genes revealed the diversity of their functions and the complexity of soybean fatty acid metabolism. Our findings provide important information for research on the fatty acid metabolism pathway in soybean. Furthermore, our results will help identify candidate genes for potential fatty acid-profile modifications to improve soybean seed oil content.ConclusionsThis is the first time that in silico studies have been used to report the genomic and proteomic characteristics of DGAT in soybean and the effect of its specific expression on organs, age, and stages.
Journal	Peerj
Volume Number	11
PubMed Central reference number	PMC10035420
PubMed reference number	36968000
e-ISSN	21678359
DOI	10.7717/peerj.14941
Language	English
Publisher	PeerJ Inc.
Publisher Date	2023-03-20
Publisher Place	San Diego, USA
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits using, remixing, and building upon the work non-commercially, as long as it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. ©2023 Zhao et al.
Subject Keyword	Soybean Diacylglycerol acyltransferase Phylogenetic analysis Genome-wide expression analysis
Content Type	Text
Resource Type	Article
Subject	Neuroscience Medicine Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology