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The root meristem growth factor BrRGF6 positively regulates Chinese cabbage to infection of clubroot disease caused by Plasmodiophora Brassicae.
| Content Provider | Europe PMC |
|---|---|
| Author | Ge, Wenjie Zhang, Jing Feng, Hui Wang, Yilian Ji, Ruiqin |
| Copyright Year | 2023 |
| Abstract | Chinese cabbage has a high annual demand in China. However, clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield. Transcriptome analysis identified a root meristem growth factor 6 (BrRGF6) as significantly up-regulated in Chinese cabbage roots infected with Plasmodiophora brassicae. Quantitative reverse-transcription polymerase chain reaction and in situ hybridization analysis showed higher BrRGF6 expression in susceptible materials than in resistant materials. After Plasmodiophora brassicae infection, BrRGF6 expression was significantly up-regulated, especially in susceptible materials. Gene function analysis showed that the roots of Arabidopsis mutant rgf6 grew faster than the wild-type, and delayed the infection progress of Plasmodiophora brassicae. A Protein, nuclear transcription factor Y subunit C (BrNF-YC), was screened from yeast two-hybrid library of Chinese cabbage induced by Plasmodiophora brassicae, and verified to interact with BrRGF6 by yeast two-hybrid co-transfer. Yeast one-hybrid and β-Glucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of BrRGF6. Transgenic verification showed that BrRGF6 or BrNF-YC silenced Chinese cabbage significantly decreased the expression of BrRGF6, accelerated root development, and reduced incidence of clubroot disease. However, after overexpression of BrRGF6 or BrNF-YC, the phenotype showed a reverse trend. Therefore, BrRGF6 silencing accelerated root growth and enhanced resistance to clubroot disease, which was regulated by BrNF-YC. |
| Page Count | 13 |
| ISSN | 26626810 |
| Journal | Horticulture Research |
| Volume Number | 10 |
| PubMed Central reference number | PMC10018783 |
| Issue Number | 3 |
| PubMed reference number | 36938571 |
| e-ISSN | 20527276 |
| DOI | 10.1093/hr/uhac292 |
| Language | English |
| Publisher | Oxford University Press |
| Publisher Date | 2022-12-30 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Plant Science Biochemistry Horticulture Biotechnology |
