Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.

Content Provider	Europe PMC
Author	HIURA, Koki MARUYAMA, Takumi WATANABE, Masaki NAKANO, Kenta OKAMURA, Tadashi SASAKI, Hayato SASAKI, Nobuya
Copyright Year	2023
Abstract	Inflammatory bowel disease (IBD) is classified into two types: Crohn’s disease andulcerative colitis. In IBD, the imbalance between the pro-inflammatory andanti-inflammatory cytokines prevents recovery from the inflammatory state, resulting inchronic inflammation in the colon. The mitotic spindle positioning protein (MISP) islocalized to the apical membrane in the colon. In this study, we observed increasedexpression of MISP in the intestinal epithelial cells in dextran sulfate sodium(DSS)-induced colitis in mice. MISP-deficient mice receiving DSS showed significantexacerbation of colitis (e.g., weight loss, loss of the crypts). The intestinal epithelialcells of the MISP-deficient mice showed a trend towards decreased cell proliferation afterDSS treatment. Reverse transcription followed by quantitative polymerase chain reactionrevealed that the expression levels of Tgfb1, an anti-inflammatorycytokine, were significantly reduced in the colon of MISP-deficient mice compared with thewild-type mice regardless of DSS treatment. These findings indicate that MISP may play arole in the recovery of the colon after inflammation through its anti-inflammatory andproliferative activities, suggesting that MISP may be a new therapeutic target forIBD.
ISSN	09167250
Volume Number	85
PubMed Central reference number	PMC10017287
Issue Number	2
PubMed reference number	36596561
Journal	The Journal of Veterinary Medical Science [J. Vet. Med. Sci]
e-ISSN	13477439
DOI	10.1292/jvms.22-0483
Language	English
Publisher	The Japanese Society of Veterinary Science
Publisher Date	2022-12-30
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the CreativeCommons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0:https://creativecommons.org/licenses/by-nc-nd/4.0/) ©2023 The Japanese Society of Veterinary Science
Subject Keyword	dextran sulfate sodium-induced colitis inflammatory bowel disease inflammatory cytokines mitotic spindle positioning protein
Content Type	Text
Resource Type	Article
Subject	Veterinary