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Data from: Development of a Nasonia vitripennis outbred laboratory population for genetic analysis (Dataset)
| Content Provider | Dryad |
|---|---|
| Author | van de Zande, Louis Ferber, Steven de Haan, Ammerins Beukeboom, Leo W. van Heerwaarden, Joost Pannebakker, Bart A. |
| Abstract | The parasitoid wasp genus Nasonia has rapidly become a genetic model system for developmental and evolutionary biology. The release of its genome sequence led to the development of high-resolution genomic tools, for both interspecific and intraspecific research, which has resulted in great advances in understanding Nasonia biology. To further advance the utility of Nasonia vitripennis as a genetic model system and to be able to fully exploit the advantages of its fully sequenced and annotated genome, we developed a genetically variable and well-characterized experimental population. In this study, we describe the establishment of the genetically diverse HVRx laboratory population from strains collected from the field in the Netherlands. We established a maintenance method that retains genetic variation over generations of culturing in the laboratory. As a characterization of its genetic composition, we provide data on the standing genetic variation and estimate the effective population size (Ne) by microsatellite analysis. A genome-wide description of polymorphism is provided through pooled resequencing, which yielded 417 331 high-quality SNPs spanning all five Nasonia chromosomes. The HVRx population and its characterization are freely available as a community resource for investigators seeking to elucidate the genetic basis of complex trait variation using the Nasonia model system. |
| File Size | 30219155 |
| File Format | HTM / HTML |
| ISSN | 17550998 |
| DOI | 10.5061/dryad.f2330 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86256 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86258 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86260 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86262 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86264 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86257 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86259 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86261 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86263 |
| Alternate Webpage(s) | https://datadryad.org/stash/downloads/file_stream/86265 |
| Language | English |
| Publisher Date | 2013-11-12 |
| Access Restriction | Open |
| Subject Keyword | Parasitoid Wasp Single Nucleotide Polymorphism (snp) Nasonia Vitripennis Pooled Resequencing Laboratory Strain |
| Content Type | Text |
| Resource Type | Data Set |
| Subject | Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
