NDLI: A nuclear genome assembly of an extinct flightless bird, the little bush moa (Dataset)

Content Provider	Dryad
Author	Cloutier, Alison Edwards, Scott Cockburn, Glenn Driver, Robert Grayson, Phil Katoh, Kazutaka Baldwin, Maude Sackton, Timothy Baker, Allan
Abstract	We present a draft genome of the little bush moa (Anomalopteryx didiformis) - one of approximately nine species of extinct flightless birds from Aotearoa, New Zealand - using ancient DNA recovered from a fossil bone from the South Island. We recover a complete mitochondrial genome at 249.9X depth of coverage and almost 900 Mb of a male moa nuclear genome at ~4-5X coverage, with sequence contiguity sufficient to identify more than 85% of avian universal single-copy orthologs. We describe a diverse landscape of transposable elements and satellite repeats; estimate a long-term effective population size of ~240,000; identify a diverse suite of olfactory receptor genes and an opsin repertoire with sensitivity in the UV range; show that the wingless moa phenotype is likely not attributable to gene loss or pseudogenization; and identify potential function-altering coding sequence variants in moa that could be synthesized for future functional assays. This genomic resource should support further studies of avian evolution and morphological divergence.
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File Format	HTM / HTML
ISSN	23752548
DOI	10.5061/dryad.d51c59zxp
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Language	English
Publisher Date	2024-04-01
Access Restriction	Open
Subject Keyword	Little Bush Moa Ancient Dna New Zealand Ratite Flightless Microsatellite
Content Type	Text
Resource Type	Data Set
Subject	Multidisciplinary

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