NDLI: Raw microscopy data of split-GFP and immunofluorescence experiments (Dataset)

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Raw microscopy data of split-GFP and immunofluorescence experiments (Dataset)

Content Provider	Dryad
Author	Peker, Esra Weiss, Konstantin Julian Riemer, Jan
Abstract	Mitochondria critically rely on protein import and its tight regulation. Here, we found that the complex I assembly factor NDUFAF8 follows a two-step import pathway linking IMS and matrix import systems. A weak targeting sequence drives TIM23-dependent NDUFAF8 matrix import, and en route allows exposure to the IMS disulphide relay which oxidizes NDUFAF8. Import is closely surveyed by proteases: YME1L prevents accumulation of excess NDUFAF8 in the IMS, while CLPP degrades reduced NDUFAF8 in the matrix. Therefore, NDUFAF8 can only fulfil its function in complex I biogenesis if both oxidation in the IMS and subsequent matrix import work efficiently. We propose that the two-step import pathway for NDUFAF8 allows to integrate the activity of matrix complex I biogenesis pathways with the activity of the mitochondrial disulphide relay system in the IMS. Such coordination might not be limited to NDUFAF8 as we identified further proteins that can follow such a two-step import pathway. The raw microscopy data provided in this dataset were obtained to visualize the localization of NDUFAF8 and further proteins following a two-step import pathway.
File Size	1118123091
File Format	HTM / HTML
ISSN	00219525
DOI	10.5061/dryad.0zpc8672t
Alternate Webpage(s)	https://datadryad.org/stash/downloads/file_stream/2274419
Alternate Webpage(s)	https://datadryad.org/stash/downloads/file_stream/2274420
Alternate Webpage(s)	https://datadryad.org/stash/downloads/file_stream/2274423
Alternate Webpage(s)	https://datadryad.org/stash/downloads/file_stream/2274421
Alternate Webpage(s)	https://datadryad.org/stash/downloads/file_stream/2274422
Language	English
Publisher Date	2023-05-04
Access Restriction	Open
Subject Keyword	Confocal Microscopy Cell Biology
Content Type	Text
Resource Type	Data Set
Subject	Cell Biology Medicine

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