NDLI: Degradation of Herpes Simplex Virus-1 Viral miRNA H11 by Vaccinia Virus Protein VP55 Attenuates Viral Replication

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Weixuan Zou Xusha Zhou Lei Wang Grace Guoying Zhou Xiaoqing Chen
Abstract	Among 29 distinct miRNAs expressed by the herpes simplex virus-1 (HSV-1) during lytic infection, miR-H11, together with miR-H1 to miR-H8 are reported to locate in the RNA-induced silencing complex (RISC). miR-H11 is encoded within viral origins of replication and lies entirely within the origins of replication. However, the roles of this miRNA derived from lytic infection with HSV-1 remain unclear. Using the advantage of vaccinia virus protein VP55 (VP55)-mediated degradation of miRNAs, we constructed a recombinant virus expressing VP55 (R5502) to demonstrate that: (1) accumulation of miR-H11 from R5502 was reduced by 540-fold versus that in cells infected with wild-type HSV-1, but miR-H1 to miR-H8 which also located in the RISC were not reduced significantly from R5502 compare with wild-type HSV-1; (2) downregulation of miR-H11 from R5502 infected cells results in markedly lower viral DNA synthesis compared with wild-type HSV-1; and (3) downregulation of miR-H11 also restricted viral spreading, and resulted in low accumulation of representative viral proteins and viral yields. The findings were confirmed through either using of a miR-H11 inhibitor or pre-transfection of a plasmid expressing VP55. These data suggest that miR-H11 plays a currently unidentified role in maintaining sufficient viral DNA synthesis during the course of viral infection.
e-ISSN	1664302X
DOI	10.3389/fmicb.2020.00717
Journal	Frontiers in Microbiology
Volume Number	11
Language	English
Publisher	Frontiers Media S.A.
Publisher Date	2020-04-01
Publisher Place	Switzerland
Access Restriction	Open
Subject Keyword	Microbiology Mir-h11 Vp55 Dna Synthesis Replication Herpes Simplex Virus-1
Content Type	Text
Resource Type	Article

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