Simultaneous determination of four sedative-hypnotics in human urine based on dendritic structured magnetic nanomaterials

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Xin Wang Hongyan Ren Jing An Weijun Kang Tangjuan Zhao Haojing Song Hongmei Shi Lingmei Niu Zhanjun Dong
Abstract	A magnetic solid-phase extraction technique based on magnetic dendritic structured nanoparticles (Fe3O4@SiO2-NH2-G5) as adsorbent coupled with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) has been developed to detect diazepam, midazolam, zolpidem, and zaleplon in human urine. With Fe3O4@SiO2-NH2 as the central core, dendrimer (G5) grafted alternately with cyanuric chloride and imidazole were bonded to the surface of the core to synthesize Fe3O4@SiO2-NH2-G5. The morphology and structure of the magnetic materials were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and dynamic light scattering (DLS). The key parameters affecting the extraction efficiency were optimized. A satisfactory performance was obtained under the optimum extraction conditions. The proposed method was validated, and the limits of detection of zaleplon, diazepam, zolpidem, and midazolam were 0.05, 0.05, 0.02, and 0.02 ng mL−1, respectively. The linear correlation coefficients r of the four analytes were > 0.996, the intra-day precision was between 2.4 % and 9.4 % with the recoveries between 88.3 % and 104.8 %, and the inter-day precision ranged from 3.9 % to 15.2 % with the recovery in the range of 94.1 %−108.3 %. The magnetic dendritic structure nanomaterial Fe3O4@SiO2-NH2-G5 was successfully used to extract sedative-hypnotic drugs from human urine samples. The Fe3O4@SiO2-NH2-G5-based magnetic solid-phase extraction method eliminates centrifugation and filtration steps as in conventional extraction. Only one step of vortex dispersion extraction could achieve the separation and purification of the target compounds. The proposed method was simple, rapid, environment-friendly, and suitable for the analysis of sedative-hypnotic drugs in human urine.
Related Links	http://www.sciencedirect.com/science/article/pii/S1878535222006797
ISSN	18785352
DOI	10.1016/j.arabjc.2022.104363
Journal	Arabian Journal of Chemistry
Issue Number	12
Volume Number	15
Language	English
Publisher	Elsevier
Publisher Date	2022-01-01
Publisher Place	Netherlands
Access Restriction	Open
Subject Keyword	Chemistry Magnetic Solid-phase Extraction Dendritic Structured Nanomaterials Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry Sedative-hypnotics Human Urine
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering