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| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Scott T. Moen Christopher L. Hatcher Anup K. Singh |
| Abstract | We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing. |
| e-ISSN | 19326203 |
| DOI | 10.1371/journal.pone.0153137 |
| Journal | PLoS ONE |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | Public Library of Science (PLoS) |
| Publisher Date | 2016-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Medicine Science |
| Content Type | Text |
| Resource Type | Article |
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