NDLI: Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

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Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Guo-quan MI Liu-sheng DUAN Zhen-xian ZHANG Li-ying LIU Hua-zhong REN Jia-chang ZHANG Xiao-lan ZHANG
Abstract	Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOS5 gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.
Related Links	http://www.sciencedirect.com/science/article/pii/S2095311913602701
ISSN	20953119
e-ISSN	23523425
DOI	10.1016/S2095-3119(13)60270-1
Journal	Journal of Integrative Agriculture
Issue Number	5
Volume Number	12
Language	English
Publisher	Elsevier
Publisher Date	2013-01-01
Publisher Place	Netherlands
Access Restriction	Open
Subject Keyword	Agriculture (general) Aba Low-temperature Stress Los5 Transgenic Plant Cucumber Salt Stress
Content Type	Text
Resource Type	Article
Subject	Ecology Plant Science Biochemistry Animal Science and Zoology Food Science Agronomy and Crop Science Food Animals

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