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Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital
| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Jayanthi Siva Subramaniyan Jeya Meenakshi Sundaram |
| Abstract | CONTEXT: ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. Pseudomonas aeruginosa and Acinetobacter species are "very successful" pathogen and the emergence of the Metallo-β-Lactamases (MBL) is becoming a therapeutic challenge. AIMS: To isolate the Nonfermenting Gram negative bacilli from the ICU samples. To identify the metallo betalactamase producers and to detect the bla gene presence among the Pseudomonas aeruginosa and Acinetobacter baumannii. SETTINGS AND DESIGN: The Nonfermenting Gram negative bacilli isolates from the ICU samples were taken over for 5 years (2009-2014) in a tertiary care hospital. METHODS AND MATERIALS: The isolates of Pseudomonas species and Acinetobacter species were confirmed by API analyser and processed according to standard procedures. Detection of the MBL producers were done by E strip method and subjected for bla gene detection by PCR method. RESULTS: In our study a total of 195 isolates of NFGNB were obtained from various ICU. Of these MBL producers, 26 % were Pseudomonas aeruginosa and 25 % were Acinetobacter baumannii. The subtypes of bla VIMMBL producing P.aeruginosa were 26%.The predominant gene coding for MBL activity in A.baumannii were found to be bla OXA gene 11.9%. The gene accession numbers were KF975367, KF975372. CONCLUSIONS: We have to control the development and dissemination of these superbugs among the ICU's. |
| Related Links | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_108_17 |
| ISSN | 09742727 |
| e-ISSN | 09747826 |
| DOI | 10.4103/JLP.JLP_108_17 |
| Journal | Journal of Laboratory Physicians |
| Issue Number | 02 |
| Volume Number | 10 |
| Language | English |
| Publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| Publisher Date | 2018-01-01 |
| Publisher Place | India |
| Access Restriction | Open |
| Subject Keyword | Medicine Acinetobacter Baumanni Bla Genes Icu Metallo-β-lactamases Pseudomonas Aeruginosa |
| Content Type | Text |
| Resource Type | Article |
