NDLI: Assessment of transcriptional importance of cell line-specific features based on GTRD and FANTOM5 data.

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Ruslan N. Sharipov Yury V. Kondrakhin Anna S. Ryabova Ivan S. Yevshin Fedor A. Kolpakov
Abstract	Creating a complete picture of the regulation of transcription seems to be an urgent task of modern biology. Regulation of transcription is a complex process carried out by transcription factors (TFs) and auxiliary proteins. Over the past decade, ChIP-Seq has become the most common experimental technology studying genome-wide interactions between TFs and DNA. We assessed the transcriptional significance of cell line-specific features using regression analysis of ChIP-Seq datasets from the GTRD database and transcriptional start site (TSS) activities from the FANTOM5 expression atlas. For this purpose, we initially generated a large number of features that were defined as the presence or absence of TFs in different promoter regions around TSSs. Using feature selection and regression analysis, we identified sets of the most important TFs that affect expression activity of TSSs in human cell lines such as HepG2, K562 and HEK293. We demonstrated that some TFs can be classified as repressors and activators depending on their location relative to TSS.
e-ISSN	19326203
DOI	10.1371/journal.pone.0243332
Journal	PLoS ONE
Issue Number	12
Volume Number	15
Language	English
Publisher	Public Library of Science (PLoS)
Publisher Date	2020-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Medicine Science
Content Type	Text
Resource Type	Article

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