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| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Willem Jan R. Fokkink David Falck Tom C. M. Santbergen Ruth Huizinga Manfred Wuhrer Bart C. Jacobs |
| Abstract | Intravenous immunoglobulin (IVIg) products from different pharmaceutical companies vary in composition, in part because of the selected blood donors and production process. N-glycosylation of the Fc-portion of IgG varies between blood donors and may influence both the side-effects and therapeutic effectiveness of IVIg. At present, the variation in Fc N-glycosylation between IVIg products has not been defined. Utilizing mass spectrometry, we performed relative quantitation of the Fc N-glycosylation of IgG, assessing a total of 154 unique lot numbers of IVIg. Seven products showed comparable Fc N-glycosylation, with only one product differing from the others in all glycosylation features (galactosylation, sialylation, fucosylation and bisecting N-acetylglucosamine). However, the mean difference did not exceed 3%. Within product variation was present to a minor degree, but largely indistinguishable from analytical variation. In conclusion, we expect that the minor variation in Fc N-glycosylation between IVIg products has a small effect, if any, on the biological activity. |
| e-ISSN | 19326203 |
| DOI | 10.1371/journal.pone.0139828 |
| Journal | PLoS ONE |
| Issue Number | 10 |
| Volume Number | 10 |
| Language | English |
| Publisher | Public Library of Science (PLoS) |
| Publisher Date | 2015-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Medicine Science |
| Content Type | Text |
| Resource Type | Article |
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