Experimental evidence for the benefits of higher X-ray energies for macromolecular crystallography

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Selina L. S. Storm Danny Axford Robin L. Owen
Abstract	X-ray-induced radiation damage is a limiting factor for the macromolecular crystallographer and data must often be merged from many crystals to yield complete data sets for the structure solution of challenging samples. Increasing the X-ray energy beyond the typical 10–15 keV range promises to provide an extension of crystal lifetime via an increase in diffraction efficiency. To date, however, hardware limitations have negated any possible gains. Through the first use of a cadmium telluride EIGER2 detector and a beamline optimized for high-energy data collection, it is shown that at higher energies fewer crystals will be required to obtain complete data, as the diffracted intensity per unit dose increases by a factor of more than two between 12.4 and 25 keV. Additionally, these higher energy data can provide more information, as shown by a systematic increase in the high-resolution cutoff of the data collected. Taken together, these gains point to a high-energy future for synchrotron-based macromolecular crystallography.
Related Links	http://scripts.iucr.org/cgi-bin/paper?S2052252521008423
ISSN	20522525
DOI	10.1107/S2052252521008423
Journal	IUCrJ
Issue Number	6
Volume Number	8
Language	English
Publisher	International Union of Crystallography
Publisher Date	2021-01-01
Publisher Place	United Kingdom
Access Restriction	Open
Subject Keyword	Crystallography High-energy X-rays Macromolecular Crystallography X-ray Radiation Damage
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry Condensed Matter Physics Materials Science