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| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Noemi Molina Angela Martin-Serrano Tahia D. Fernandez Amene Tesfaye Francisco Najera María J. Torres Cristobalina Mayorga Yolanda Vida Maria I. Montañez Ezequiel Perez-Inestrosa |
| Abstract | Dendrimeric Antigens (DeAns) consist of dendrimers decorated with multiple units of drug antigenic determinants. These conjugates have been shown to be a powerful tool for diagnosing penicillin allergy using in vitro immunoassays, in which they are recognized by specific IgE from allergic patients. Here we propose a new diagnostic approach using DeAns in cellular tests, in which recognition occurs through IgE bound to the basophil surface. Both IgE molecular recognition and subsequent cell activation may be influenced by the tridimensional architecture and size of the immunogens. Structural features of benzylpenicilloyl-DeAn and amoxicilloyl-DeAn (G2 and G4 PAMAM) were studied by diffusion Nuclear Magnetic Resonance (NMR) experiments and are discussed in relation to molecular dynamics simulation (MDS) observations. IgE recognition was clinically evaluated using the basophil activation test (BAT) for allergic patients and tolerant subjects. Diffusion NMR experiments, MDS and cellular studies provide evidence that the size of the DeAn, its antigen composition and tridimensional distribution play key roles in IgE-antigen recognition at the effector cell surface. These results indicate that the fourth generation DeAns induce a higher level of basophil activation in allergic patients. This approach can be considered as a potential complementary diagnostic method for evaluating penicillin allergy. |
| e-ISSN | 14203049 |
| DOI | 10.3390/molecules23050997 |
| Journal | Molecules |
| Issue Number | 5 |
| Volume Number | 23 |
| Language | English |
| Publisher | MDPI AG |
| Publisher Date | 2018-04-01 |
| Publisher Place | Switzerland |
| Access Restriction | Open |
| Subject Keyword | Organic Chemistry Pamam Dendrimeric Antigens Penicillin Drug Allergy Basophil Activation Tests |
| Content Type | Text |
| Resource Type | Article |
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