NDLI: Direct identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) from positive blood culture bottles: An opportunity to customize growth conditions for fastidious organisms causing bloodstream infections

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Direct identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) from positive blood culture bottles: An opportunity to customize growth conditions for fastidious organisms causing bloodstream infections

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Megha Sharma Vikas Gautam Monika Mahajan Sudesh Rana Manasi Majumdar Pallab Ray
Abstract	Culture-negative bacteraemia has been an enigmatic entity with respect to its aetiological agents. In an attempt to actively identify those positive blood cultures that escape isolation and detection on routine workflow, an additional step of MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) based detection was carried out directly from the flagged blood culture bottles. Blood samples from 200 blood culture bottles that beeped positive with automated (BACTEC) system and showed no growth of organism on routine culture media, were subjected to analysis by MALDI-TOF MS. Forty seven of the 200 (23.5%) bacterial aetiology could be established by bottle-based method. Based on these results, growth on culture medium could be achieved for the isolates by providing special growth conditions to the fastidious organisms. Direct identification by MALDI-TOF MS from BACTEC-positive bottles provided an opportunity to isolate those fastidious organisms that failed to grow on routine culture medium by providing them with necessary alterations in growth environment.
Related Links	http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=146;issue=4;spage=541;epage=544;aulast=Sharma
ISSN	09715916
DOI	10.4103/ijmr.IJMR_823_16
Journal	Indian Journal of Medical Research
Issue Number	4
Volume Number	146
Language	English
Publisher	Wolters Kluwer Medknow Publications
Publisher Date	2017-01-01
Publisher Place	India
Access Restriction	Open
Subject Keyword	Medicine Culture-negative Bacteraemia - Fastidious Organisms - Maldi-tof Ms
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry, Genetics and Molecular Biology

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