NDLI: Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions

Content Provider	Directory of Open Access Journals (DOAJ)
Author	M. BURSTEIN H.R. SCHOLNICK R. MORFIN
Abstract	Procedures are described for the isolation of lipoproteins from human serum by precipitation with polyanions and divalent cations. A mixture of low and very low density lipoproteins can be prepared without ultracentrifugation by precipitation with heparin and either MnCl2 alone or MgCl2 plus sucrose. In both cases the precipitation is reversible, selective, and complete. The highly concentrated isolated lipoproteins are free of other plasma proteins as judged by immunological and electrophoretic methods. The low density and very low density lipoproteins can then be separated from each other by ultracentrifugation. The advantage of the method is that large amounts of lipoproteins can be prepared with only a single preparative ultracentrifugation. Polyanions other than heparin may also be used; when the precipitation of the low and very low density lipoproteins is achieved with dextran sulfate and MnCl2, or sodium phosphotungstate and MgCl2, the high density lipoproteins can subsequently be precipitated by increasing the concentrations of the reagents. These lipoproteins, containing small amounts of protein contaminants, are further purified by ultracentrifugation at d 1.22. With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum.
ISSN	00222275
e-ISSN	15397262
DOI	10.1016/S0022-2275(20)42943-8
Journal	Journal of Lipid Research
Issue Number	6
Volume Number	11
Language	English
Publisher	Elsevier
Publisher Date	1970-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Biochemistry Heparin Dextran Sulfate Sodium Phosphotungstate Divalent Cations Sucrose Immunology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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