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| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Maria eAgallou Evita eAthanasiou Olga eKoutsoni Eleni eDotsika Evdokia eKaragouni |
| Abstract | Leishmaniasis is a significant worldwide health problem for which no vaccine exists. Activation of CD4+ and CD8+ T cells is crucial for the generation of protective immunity against parasite. Recent trend in vaccine design has been shifted to epitope-based vaccines that are more specific, safe, and easy to produce. In the present study, four known antigenic Leishmania (L.) infantum proteins, CPA, histone H1, KMP-11 and LeIF were analysed for the prediction of binding epitopes to H2d MHC class I and class II molecules, using online available algorithms. Based on in silico analysis, eight peptides including highly scored MHC class I- and class II-restricted epitopes were synthesized. Peptide immunogenicity was validated in MHC compatible BALB/c mice immunized with each synthetic peptide emulsified in CFA/IFA. CPA_p2, CPA_p3, H1_p1 and LeIF_p6 induced strong spleen cell proliferation upon in vitro peptide re-stimulation. In addition, the majority of the peptides, except of LeIF_p1 and KMP-11_p1, induced IFN-γ secretion, while KMP-11_p1 indicated a suppressive effect on IL-10 production. CPA_p2, CPA_p3, LeIF_p3 and LeIF_p6 induced IFN-γ-producing CD4+ T cells indicating a TH1 type response. In addition, CPA_p2, CPA_p3 and H1_p1 induced also the induction of CD8+ T cells. The induction of peptide-specific IgG in immunized mice designated also the existence of B cell epitopes in peptide sequences. Combining immunoinformatic tools and experimental validation, we demonstrated that CPA_p2, CPA_p3, H1_p1, H1_p3, CPA_p2, LeIF_p3 and LeIF_p6 are likely to include potential epitopes for the induction of protective cytotoxic and/or TH1-type immune responses supporting the feasibility of peptide-based vaccine development for leishmaniasis. |
| e-ISSN | 16643224 |
| DOI | 10.3389/fimmu.2014.00268 |
| Journal | Frontiers in Immunology |
| Volume Number | 5 |
| Language | English |
| Publisher | Frontiers Media S.A. |
| Publisher Date | 2014-06-01 |
| Publisher Place | Switzerland |
| Access Restriction | Open |
| Subject Keyword | Immunologic Diseases. Allergy Histone H1 Lymphocyte Proliferation in Silico Analysis Cysteine Peptidase A Kinetoplastid Membrane Protein 11 Leishmania Eukaryotic Initiation Factor |
| Content Type | Text |
| Resource Type | Article |
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