NDLI: EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

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EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

Content Provider	Directory of Open Access Journals (DOAJ)
Author	AKBARI M. NIKBAKHT M. SOBHANI A.GH
Abstract	Introduction: Bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. Material and Methods: The stromal cells of bone marrow obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. The cells were cultured in either DMEM medium containing 15% fetal calf serum and antibiotics as the controls or the above medium supplemented with osteogenic supplements (OS): include 10 mM Na-beta glycerophosphate (Na-betaGp), 10 nM dexamethasone (Dex) and 50 g/ml ascordic acid (AsA) as the examined cultures. After 6, 12 and 18 days of grow up in subculture, the cultures were examined for mineralization and alkaline phosphatase (Apase) expression. Results: Mesenchymal stem cells (MSCs) in examined cultures underwent a dramatic change in cellular morphology and a significat increase in Apase activity by day 12. The deposition of a calcified matrix on the surface of the culture flasks became evident between days 12 and 18. Conclusion: The addition of osteogenic supplements (OS) to MSCs cultures induced Apase expression that contributes to cellular differentiation and mineralization of extracellular matrix.
Related Links	http://celljournal.org/library/upload/article/131-136.PDF
ISSN	22285806
e-ISSN	22285814
Journal	Cell Journal
Issue Number	3
Volume Number	3
Language	English
Publisher	Royan Institute (ACECR), Tehran
Publisher Date	2001-01-01
Publisher Place	Iran
Access Restriction	Open
Subject Keyword	Medicine Science Alkaline Phosphatase Bone Marrow Mesenchymal Stem Cells (mscs) Osteogenesis Culture Rat
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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