Immunohistochemical study of $p16^{ INK4A}$ and survivin expressions in cervical squamous neoplasm

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Tan Geok Sharifah N. Hatta A. Paul-Ng H. Razmin Ghazali Shiran M. Norlatiffah Sydee
Abstract	Introduction:Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of $p16^{INK4A}$ and survivin as possible predictive biomarkers in cervical squamous neoplasm. Material and Methods: This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using $p16^{INK4A}$ and survivin monoclonal antibodies. The $p16^{ INK4A}$ expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense. Results: It is seen that $p16^{ INK4A}$ expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed $p16^{ INK4A }$ expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and $p16^{ INK4A}$ expressions. Conclusion: Our study showed that $p16^{ INK4A}$ expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC.
Related Links	http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2010;volume=53;issue=1;spage=1;epage=6;aulast=Tan
ISSN	03774929
e-ISSN	09745130
Journal	Indian Journal of Pathology and Microbiology
Issue Number	1
Volume Number	53
Language	English
Publisher	Wolters Kluwer Medknow Publications
Publisher Date	2010-01-01
Publisher Place	India
Access Restriction	Open
Subject Keyword	Pathology Microbiology Cervix Cervical Cancer Immunohistochemistry P16 Survivin
Content Type	Text
Resource Type	Article
Subject	Pathology and Forensic Medicine Microbiology (medical)