NDLI: Squalene Cyclases and Cycloartenol Synthases from Polystichum polyblepharum and Six Allied Ferns

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Junichi Shinozaki Takahisa Nakene Akihito Takano
Abstract	Ferns are the most primitive of all vascular plants. One of the characteristics distinguishing them from flowering plants is its triterpene metabolism. Most cyclic triterpenes in ferns are hydrocarbons derived from the direct cyclization of squalene by squalene cyclases (SCs). Both ferns and more complex plants share sterols and biosynthetic enzymes, such as cycloartenol synthases (CASs). Polystichum belongs to Dryopteridaceae, and is one of the most species-rich of all fern genera. Several Polystichum ferns in Japan are classified as one of three possible chemotypes, based on their triterpene profiles. In this study, we describe the molecular cloning and functional characterization of cDNAs encoding a SC (PPH) and a CAS (PPX) from the type species Polystichum polyblepharum. Heterologous expression in Pichia pastoris revealed that PPH and PPX are hydroxyhopane synthase and CAS, respectively. By using the PPH and PPX sequences, we successfully isolated SC- and CAS-encoding cDNAs from six Polystichum ferns. Phylogenetic analysis, based on SCs and oxidosqualene cyclase sequences, suggested that the Polystichum subclade in the fern SC and CAS clades reflects the chemotype—but not the molecular phylogeny constructed using plastid molecular markers. These results show a possible relation between triterpenes and their biosynthetic enzymes in Polystichum.
e-ISSN	14203049
DOI	10.3390/molecules23081843
Journal	Molecules
Issue Number	8
Volume Number	23
Language	English
Publisher	MDPI AG
Publisher Date	2018-07-01
Publisher Place	Switzerland
Access Restriction	Open
Subject Keyword	Organic Chemistry Squalene Cyclase Cycloartenol Synthase Triterpene Fern Polystichum
Content Type	Text
Resource Type	Article

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