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Evaluation of Antibacterial and Antibiofilm Activity of New Antimicrobials as an Urgent Need to Counteract Stubborn Multidrug-resistant Bacteria
| Content Provider | Directory of Open Access Journals (DOAJ) |
|---|---|
| Author | Ahmed R. Sofy Akram A. Aboseidah El-Shahat El-Morsi Heba A. Azmy Ahmed A. Hmed |
| Abstract | The biggest problem in the world today is antimicrobial resistance, which undermines human health and increases morbidity and mortality associated with life-threatening serious diseases. Scientists from different fields are therefore examining plants for their antimicrobial use in the face of multidrug-resistant bacteria with a new eye. So, the aim of this research to find a new antibacterial from five aqueous extracts of Nigella sativa, Ziziphus spina-christi, Rosmarinus officinalis, Origanum majorana, and Allium sativum medicinal plants tested against the most multidrug-resistant bacterial isolates from clinical specimens which were Klebsiella pneumoniae (Gram-negative), Escherichia coli (Gram-negative), Pseudomonas aeruginosa (Gram-negative), Staphylococcus aureus (Gram-positive) and Methicillin-resistant Staphylococcus aureus (Gram-positive). Also, to investigate the antibiofilm activity of the most potent extract. From all tested plants, the total extract of Z. spina-christi appeared a strong antibacterial effect against all tested MDR-strains. Besides, its polyphenol fraction showed a stronger effect. Furthermore, the total extract MIC ranged between 3.125-12.5 mg/ml with MBC was 3.125-25 mg/ml against previous strains. While, polyphenol fraction MIC and MBC were about 0.312- 1.25 mg/ml and 0.312-2.5 mg/ml, respectively. In contrast to the antibacterial strength of polyphenol fraction, the antibiofilm effect was weaker than the total extract antibiofilm effect, where at ½ MIC, the reduction of biofilm was about 78.18%, 81.9% and 99.48% against S. aureus, MRSA and P. aeruginosa, respectively. While, in the case of polyphenol fraction, the biofilm reduction effect against previous strains was 63.82%, 59.97%, and 87.95%, respectively. |
| Related Links | https://microbiologyjournal.org/evaluation-of-antibacterial-and-antibiofilm-activity-of-new-antimicrobials-as-an-urgent-need-to-counteract-stubborn-multidrug-resistant-bacteria/ |
| ISSN | 09737510 |
| e-ISSN | 2581690X |
| DOI | 10.22207/JPAM.14.1.62 |
| Journal | Journal of Pure and Applied Microbiology |
| Issue Number | 1 |
| Volume Number | 14 |
| Language | English |
| Publisher | Journal of Pure and Applied Microbiology |
| Publisher Date | 2020-01-01 |
| Publisher Place | India |
| Access Restriction | Open |
| Subject Keyword | Microbiology Herbal Extracts Medicinal Plants Antimicrobial Agent Mdr-bacteria Mrsa |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Applied Microbiology and Biotechnology Biotechnology |
