Decreased GyBAA Receptor Function in the Brain Stem during Pancreatic Regeneration in Rats

Content Provider	CUSAT-Thesis
Author	Balarama Kaimal, S. Gireesh, G. Paulose, C. S.
Abstract	Gamma amino outyric acid is a majorinhibitory neurotrarsr titter in the central nervoussystem. In the preset study sv, Have investigate(' thealteration of GABA receptor, In t he hrain stem of ratsduring pancreatic regeneration. Three groups of ratswere used for the study: sham operated, 72 It and7 days partially pancreatectonnsea. GABA was (juan-(ified by [H]GABA receptor iispiacement method.GABA receptor kin: 10, pat at i et•ers were studied byusing the binding of F'.](iAhA as ligand to the TritonX-100 treated me,i1,;-:mes a1,J displacement withunlabelled GABA. GhRA,v receptor activity wasstudied by using the [` -1 h3cuculline and displacementwith unlabellecV euculline. ;.\13A content significantlydecreased (1' < (1.(101 ) it, 0-e brain stern duringthe regeneration of pancreas. 'I hl, high affinity (IAI3Areceptor binding sho?:ed it sigii'f cant decrease in 131„.,\(P < 11.01) and K,I 1).05) n 72 h and 7 days afterpartial pancreatee 'timv. ";:flhicuculline hin(Iingshowed it signih eat, 'le ( r(, :,e in /Jn1,s and K,I(P < 0.001) in 72 h pa^.rcreaw,, mised rats when comparedwith sham wt--tt' as P,n and K,I reversed tonear sham after 7 da,s of pancreatectomv. The resultssugge,) that GAB A throur,r; ('GABA receptors inbrain Atcem has a regulatory uie during active regenerationof pancreas which will have inunense clinicalsignificance in the treatment of cliahetcs.
File Format	PDF
Language	English
Publisher	Department of Biotechnology
Access Restriction	Open
Subject Keyword	GABA GABA Receptors Brain stem Bicuculline Pancreatectomy
Content Type	Text
Resource Type	Article