NDLI: Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth

Content Provider	CSIR - National Institute of Science Communication and Policy Research (NISCPR)
Author	Chaudhari, Hemangi G. Sachdev, Dhara P. Chopade, Balu A. Kasture, Vijay M. Dhavale, Dilip. D.
Abstract	The present study was undertaken for isolation of Klebsiella strains from rhizosphere of wheat (T. aestivum), screening and characterization of these strains for in vitro indole acetic acid (IAA) production and studying the effect of these strains on plant growth under gnotobiotic conditions. Nine strains of Klebsiella were isolated from rhizosphere of wheat (var. Lokwan) and identified as K. pneumoniae by 16S rRNA gene sequencing. Six K. pneumoniae strains showed in vitro IAA production. Colorimetric analysis showed that K8 produced maximum IAA (27.5 mg l-1) in the presence of tryptophan (1 mg ml-1) at 72 h of incubation with optimum conditions as pH 8.0, 37°C and 0.5% (w/v) NaCl concentration. GC-MS analysis and IR studies confirmed presence of IAA in the cell filtrates of strain K8. Effect of six IAA producing Klebsiella strains on plant growth was studied by performing series of seed germination tests using moth bean seeds under axenic conditions and pot experiments using sterilized soil and wheat seeds (var. Lokwan). Strain K11 and K42 demonstrated increase in root length of inoculated moth beans (~ 92.71% over the control). Results of pot experiments indicated that almost all the six IAA producing Klebsiella strains significantly increased the root length and shoot height of inoculated wheat seedlings over the control. The results suggest that these are promising isolates from wheat rhizosphere and merits research on appliance of these strains in agriculture.
Ending Page	1000
Starting Page	993
ISSN	00195189
e-ISSN	09751009
Journal	Indian Journal of Experimental Biology (IJEB)
Issue Number	12
Volume Number	47
Language	English
Publisher	Council of Scientific & Industrial Research (CSIR)
Publisher Date	2009-12-01
Access Restriction	Open
Subject Keyword	Klebsiella Rhizosphere Pot experiments Wheat Seed germination test Indole Acetic Acid
Content Type	Text
Resource Type	Article
Subject	Biotechnology Cell Biology Molecular Biology

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