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| Content Provider | CSIR - National Institute of Science Communication and Policy Research (NISCPR) |
|---|---|
| Author | Agarwal, Garima Murthy, V. Krishna Thomas, Jennifer Sabat, Sasmita Kushnoor, Divyashree Devaraj, Surabhi Shantha, S. L. |
| Abstract | Rhizobium sp., isolated from Mimosa pudica, Bacillus subtilis BC1 and Escherichia coli K12 were investigated for their ability to produce plant growth hormones in a given production media (PM1 & PM2). Thin layer chromatography and high pressure liquid chromatography were performed to analyse the amount of cytokinin produced by these microorganisms. E. coli K12 produced the highest amount (0.186 mg/L) of cytokinin in PM2 medium, while B. subtilis BC1 gave the least production amount (0.0075 mg/L) in the same medium. The microbially synthesized cytokinin was then incorporated onto the solidified MS medium for the shoot development in Phillodendron xanadu, an ornamental plant. The cytokinin produced from E. coli K12 gave more number of plantlets with thick shoot and lustrous leaves having 3.0 cm length for medium with charcoal (PM2); while comparatively less number of plantlets having 1.5 cm length for medium without charcoal. The cytokinin from B. subtilis BC1 and Rhizobium sp. gave comparatively more shoot number and more plant height on medium with charcoal in PM1 and PM2 respectively. |
| Ending Page | 546 |
| Starting Page | 544 |
| ISSN | 09725849 |
| e-ISSN | 09750967 |
| Journal | Indian Journal of Biotechnology (IJBT) |
| Issue Number | 04 |
| Volume Number | 13 |
| Language | English |
| Publisher | CSIR-NISCAIR, India |
| Publisher Date | 2014-10-01 |
| Access Restriction | Open |
| Rights License | CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| Subject Keyword | Bacillus subtilis Cytokinin Rhizobium sp. Shoot induction Escherichia coli Phillodendron xanadu |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology Plant Science Biotechnology Bioengineering |
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