NDLI: Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum)

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Structural characterisation and determination of prebiotic activity of purified xylo-oligosaccharides obtained from Bengal gram husk (Cicer arietinum L.) and wheat bran (Triticum aestivum)

Content Provider	CSIR - Central Food Technological Research Institute (CFTRI)
Author	Madhukumar, M. S. Muralikrishna, G.
Abstract	Water extractable polysaccharides (WEPs) were isolated from Bengal gram husk and wheat bran. These WEP were subjected to driselase enzyme hydrolysis to obtain oligosaccharide mixtures, which were purified successively on Biogel P-2 and high performance liquid chromatographies. The molecular weight and structural features of the purified oligosaccharides were deduced using ESI-MS and 1H NMR, respectively. The prebiotic properties of these purified oligosaccharides were studied by using Bifidobacterium adolescentis NDRI 236. Increase in dry cell mass (0.7-0.9mg/ml) and decrease in pH (5.8) due to production of short chain fatty acid (SCFA) indicated oligosaccharide fermentation. Acetate was the chief SCFA produced and its amount varied from 97.2% to 100%. The activities of xylanase (257-470mU/ml), xylopyranosidase (53-60mU/ml) and arabinofuranosidase (60-70mU/ml) in the culture broth indicated the breakdown of xylo-oligosaccharides and their subsequent utilisation by the bacterium for its growth.
Related Links	http://www.sciencedirect.com/science/article/B6T6R-4W74243-1/2/b8d34153a648ff1775d5e32354a9128f
Starting Page	215
Ending Page	223
Page Count	9
File Format	PDF
ISSN	03088146
Journal	Food Chemistry.
Volume Number	118
Issue Number	2
Language	English
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Legumes-Pulses Polysaccharide Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Food Science Medicine

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