Optimization of Synthesis of Ethyl Isovalerate Using Rhizomucor miehei Lipase

Content Provider	CSIR - Central Food Technological Research Institute (CFTRI)
Author	Chowdary, G. V. Prapulla, S. G.
Abstract	—Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n -hexane. Response surface methodology based on a four-variable, five-level, central composite rotatable design was employed to optimize four important reaction variables—enzyme/substrate (E/S) ratio, substrate concentration, incubation time, and temperature— affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; and temperature, 60° C. The predicted value matched well with the experimentally obtained value of 487 mM.
File Format	PDF
Language	English
Access Restriction	Authorized
Subject Keyword	Analysis Enzyme Chemistry
Content Type	Text
Resource Type	Article