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Purification and characterization of lipase from rice (Oryza sativa L.) bran
| Content Provider | CSIR - Central Food Technological Research Institute (CFTRI) |
|---|---|
| Author | Prakash, V. Rajeshwara, A. N. |
| Date of Submission | 1995-01-01 |
| Abstract | Lipase was isolated from defatted rice bran by a new procedure which yields higher enzyme activity and quantity of enzyme. The procedure involves selective ammoniuum sulphate precipitation followed by ion exchange chromatography using DEAE-Sepharose CL-6B gel and column chromatography. The purified and homogeneous enzyme was characterized for optical, physicochemical and hydrodynamic properties. The enzyme had a UV absorption max. at 276 nm with an extinction coeff. value of 15.25. Fluorescence excitation and emission max. were 285 and 344 nm, respectively. Specific activity was 18 units, and the enzyme had a pH optimum of 7.5 and temp. optimum of 30 |
| Starting Page | 406 |
| Ending Page | 418 |
| Page Count | 13 |
| File Format | |
| Issue Number | 5/6 |
| Volume Number | 39 |
| Journal | Food/Nahrung |
| Language | English |
| Access Restriction | Limited |
| Subject Keyword | Cereal Products Cereals Enzymes Lipases Rice Bran |
| Content Type | Text |
| Resource Type | Article |
