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In vitro anti-inflammatory activity among probiotic Lactobacillus species isolated from fermented foods
| Content Provider | CSIR - Central Food Technological Research Institute (CFTRI) |
|---|---|
| Author | Devi, Sundru Manjulata Kurrey, N. K. Halami, Prakash M. |
| Date of Submission | 2018-01-01 |
| Abstract | Mammalian alpha/beta hydrolase domain (ABHD) family of proteins have emerged as key regulators of lipid metabolism and are found to be associated with human diseases. Human α/β-hydrolase domain containing protein 11 (ABHD11) has recently been predicted as a potential biomarker for human lung adenocarcinoma. In silico analyses of the ABHD11 protein sequence revealed the presence of a conserved lipase motif GXSXG. However, the role of ABHD11 in lipid metabolism is not known. To understand the biological function of ABHD11, we heterologously expressed the human ABHD11 in budding yeast, Saccharomyces cerevisiae. In vivo [14C]acetate labeling of cellular lipids in yeast cells overexpressing ABHD11 showed a decrease in triacylglycerol content. Over expression of ABHD11 also alters the molecular species of triacylglycerol in yeast. Similar activity was observed in its yeast homolog, Ygr031w. The role of the conserved lipase motif in the hydrolase activity was proven by the mutation of all conserved amino acid residues of GXSXG motif. Collectively, our results demonstrate that human ABHD11 and its yeast homolog YGR031W have a pivotal role in the lipid metabolism. |
| Starting Page | 19 |
| Ending Page | 27 |
| Page Count | 9 |
| File Format | |
| ISSN | 17564646 |
| Volume Number | 47 |
| Alternate Webpage(s) | http://ir.cftri.com/13854/ |
| Journal | Journal of Functional Foods |
| Language | English |
| Publisher | Elsevier |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacteriology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Food Science Nutrition and Dietetics Medicine |
