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Chem. Senses doi:10.1093/chemse/bjq020 PI3Kg-Dependent Signaling in Mouse Olfactory Receptor Neurons (2010)
| Content Provider | CiteSeerX |
|---|---|
| Author | Corey, Elizabeth A. Brunert, Daniela Klasen, Katharina Ache, Barry W. |
| Abstract | Phosphatidylinositol 3-kinase (PI3K)-dependent signaling couples to receptors for many different ligands in diverse cellular systems. Recent findings suggest that PI3K-dependent signaling also mediates inhibition of odorant responses in rat olfactory receptor neurons (ORNs). Here, we present evidence that murine ORNs show PI3K-dependent calcium responses to odorant stimulation, they express 2 G protein-coupled receptor (GPCR)-activated isoforms of PI3K, PI3Kb and PI3Kc, and they exhibit odorant-induced PI3K activity. These findings support our use of a transgenic mouse model to begin to investigate the mechanisms underlying PI3K-mediated inhibition of odorant responses in mammalian ORNs. Mice deficient in PI3Kc, a class IB PI3K that is activated via GPCRs, lack detectable odorant-induced PI3K activity in their olfactory epithelium and their ORNs are less sensitive to PI3K inhibition. We conclude that odorant-dependent PI3K signaling generalizes to the murine olfactory system and that PI3Kc plays a role in mediating inhibition of odorant responses in mammalian ORNs. Key words: calcium imaging, complex odorants, ELISA, inhibitory input, transgenic |
| File Format | |
| Publisher Date | 2010-01-01 |
| Access Restriction | Open |
| Content Type | Text |