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Antigenic characterization of the nucleoprotein and matrix protein of influenza C virus with monoclonal antibodies (1991)
| Content Provider | CiteSeerX |
|---|---|
| Author | Sugawara, K. Nishimura, H. Hongo, S. Kitame, F. Nakamura, K. |
| Abstract | Monoclonal antibodies against the nucleoprotein (NP) and matrix (M) protein of influenza C virus were prepared and characterized. At least two non-overlap-ping or partially overlapping antigenic sites were delineated on each of the proteins by competitive binding assays. Western blot analysis howed that two antigenic sites on the M protein were highly resistant to conformational changes whereas two sites on NP were sensitive. No antigenic variation was seen in either the NP or the M protein when the reactivity of monoclonal antibodies with 23different influenza C strain sisolated over a 41 year period was studied by radioimmunopre-cipitation and enzyme-linked immunosorbent assay. Immunofluorescence analysis with the monoclonal antibodies revealed that both the NP and M proteins migrated to the cell nucleus during the replication cycle of influenza C virus. |
| File Format | |
| Publisher Date | 1991-01-01 |
| Access Restriction | Open |
| Content Type | Text |