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Direct time-resolved fluorescence immunoassay of progesterone in serum involving the biotinstreptavidin system and the immobilized antibody approach (1992)
| Content Provider | CiteSeerX |
|---|---|
| Author | Kakabakos, S. E. Khosravi, M. J. |
| Abstract | We developed a direct competitive-type immunoassay for progesteronein serum that combines the advantages of the biotin-streptavidinsystemwiththe antibody-immobi-lization approach. We synthesized biotinylatedprogester-one derivatives of five different proteins and, after initial evaluation of the conjugates, selected biotinylated bovine lgG-progesterone as the most suitable tracer. Progester-one released from binding proteins with danazol com-petes with the biotinylated tracer conjugate for binding to a limitedamount of a mouse anti-progesterone monoclo-nal antibody in microtitration wells coated with a goat anti-mouse lgG antibody. The binding of the biotinylated tracer is then monitored by reaction with a streptavidin-based universal detection reagent developed for time-resolved fluorometry. The assay demonstrated typical |
| File Format | |
| Journal | Clinical Chemistry |
| Language | English |
| Publisher Date | 1992-01-01 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |