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Efficient Intracellular Delivery of Molecules with High Cell Viability Using Nanosecond-Pulsed Laser- Activated Carbon Nanoparticles
| Content Provider | CiteSeerX |
|---|---|
| Abstract | any pharmaceutical agents in de-velopment and used clinically need to be delivered intracellu-larly to have their intended therapeutic effect.1 Inside the cell they have various targets, including nuclear targets for gene transfection, gene correction and other gene-based therapies;2 mitochondrial tar-gets for certain proapoptotic drugs;3,4 and other cytoplasmic sites, including those needed for protein knockdown by RNA interference using siRNA or miRNA.5 However, the highly structured and lipophilic nature of the cells ' plasma mem-branes generally blocks the direct intracel-lular delivery of such compounds, such that most molecules other than small ions enter cells by an active transport mechanism, such as receptor-mediated endocytosis.6 Uptake via that route can be accessed through the use of lipid and polymer nano-particles, especially if decoratedwith receptor-targeted ligands.7 However, such chemical delivery systems can be associated with cyto-toxicity and drugs administered by that route can be subject to lysosomal degradation after internalization.8 Viral vectors also harness natural mechanisms of intracellular delivery, but areprimarily useful only forDNAdelivery and suffer from risks of virus-induced toxicity.9 Another approach to intracellular deliv-ery uses physical forces to transiently and reversibly disrupt the cell membrane, * Address correspondence to |
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| Access Restriction | Open |
| Content Type | Text |
