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Fast Immobilization of Glucose Oxidase on Graphene Oxide for Highly Sensitive Glucose Biosensor Fabrication
| Content Provider | CiteSeerX |
|---|---|
| Author | Sehat, Ali Akbari Khodadadi, Abbas Ali Shemirani, Farzaneh Mortazavi, Yadollah |
| Abstract | A fast and simple immobilization of glucose oxidase (GOx) used for fabrication of highly sensitive, quick response (< 2 s) and low cost glucose biosensor. GOx enzyme was immobilized covalently on graphene oxide (GO) modified pencil graphite electrode (PGE) and simultaneously GO was reduced by chronoamperometric and cyclic voltammetric methods. The fabricated biosensor by chronoamperometry showed sharper redox peaks current for GOx in comparison with cyclic voltammetry. Attenuated transmission reflectance (ATR) spectroscopy was used for characterization of covalent immobilization of GOx. The immobilization time of GOx on GO was shortened to 30 s, the minimum immobilization time in other works was 20 minutes, after drying of GO-GOx paste on the PGE. The electrochemical properties of the fabricated biosensor were studied by cyclic voltammetry (CV) and chronoamperometry. The cyclic voltammetric behavior of the fabricated electrode shows a pair of well-defined peaks with a formal potential of-0.521 V and peak to peak separation of 35 mV, indicating direct electron transfer between GOx and electrode. The fabricated biosensor shows a fast electron transfer of ks=5.84 s-1, a high affinity for glucose with Michaelis-Menten constant Km = 0.215 mM, a high sensitivity of 278.4 µA mM-1 cm-2, a low detection limit of 0.61 µM, and a linear range of 40 to 600 µM. The fast and simple immobilization and GO reduction method can be used in fabrication of other electrochemical biosensors and biofuel cells with low costs. |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Glucose Oxidase Graphene Oxide Highly Sensitive Glucose Biosensor Fabrication Fast Immobilization Cyclic Voltammetry Simple Immobilization Minimum Immobilization Time Biofuel Cell Low Cost Glucose Biosensor Low Detection Limit Linear Range Pencil Graphite Electrode Fabricated Biosensor High Affinity Redox Peak Transmission Reflectance High Sensitivity Direct Electron Transfer Well-defined Peak Fabricated Electrode Michaelis-menten Constant Km Go Reduction Method Fast Electron Transfer Electrochemical Biosensors Covalent Immobilization Cyclic Voltammetric Method Mm-1 Cm-2 Quick Response Low Cost Cyclic Voltammetric Behavior Electrochemical Property Go-gox Paste Immobilization Time Formal Potential Of-0 Gox Enzyme |
| Content Type | Text |
