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Tumor necrosis factor-a contributes to ischemia- and reperfusioninduced endothelial activation in isolated hearts (1999)
| Content Provider | CiteSeerX |
|---|---|
| Author | Kupatt, Christian Habazettl, Helmut Goedecke, Axel Wolf, Dieter A. Zahler, Stefan Boekstegers, Peter Kelly, Ralph A. Becker, Bernhard F. |
| Abstract | Abstract—During myocardial reperfusion, polymorphonuclear neutrophil (PMN) adhesion involving the intercellular adhesion molecule-1 (ICAM-1) may lead to aggravation and prolongation of reperfusion injury. We studied the role of early tumor necrosis factor-a (TNF-a) cleavage and nuclear factor-kB (NF-kB) activation on ICAM-1 expression and venular adhesion of PMN in isolated hearts after ischemia (15 minutes) and reperfusion (30 to 480 minutes). NF-kB activation (electromobility shift assay) was found after 30 minutes of reperfusion and up to 240 minutes. ICAM-1 mRNA, assessed by Northern blot, increased during the same interval. Functional effect of newly synthesized adhesion molecules was found by quantification (in situ fluorescence microscopy) of PMN, given as bolus after ischemia, which became adherent to small coronary venules (10 to 50 mm in diameter). After 480 minutes of reperfusion, ICAM-1–dependent PMN adhesion increased 2.5-fold compared with PMN adhesion obtained during acute reperfusion. To study the influence of NF-kB on PMN adhesion, we inhibited NF-kB activation by transfection of NF-kB decoy oligonucleotides into isolated hearts using HJV-liposomes. Decoy NF-kB but not control oligonucleotides blocked ICAM-1 upregulation and inhibited the subacute increase in PMN adhesion. Similar effects were obtained using BB 1101 (10 mg), an inhibitor of TNF-a cleavage enzyme. These data suggest that ischemia and reperfusion in isolated hearts cause liberation of TNF-a, activation of NF-kB, and upregulation of ICAM-1, an adhesion molecule involved in inflammatory response after ischemia and reperfusion. (Circ Res. 1999;84:392-400.) |
| File Format | |
| Journal | Circ. Res |
| Language | English |
| Publisher Date | 1999-01-01 |
| Access Restriction | Open |
| Subject Keyword | Isolated Heart Endothelial Activation Tumor Necrosis Factor-a Contributes Pmn Adhesion Adhesion Molecule Nf-kb Activation Icam-1 Expression Similar Effect Nf-kb Decoy Oligonucleotides Polymorphonuclear Neutrophil Functional Effect Icam-1 Mrna Acute Reperfusion Reperfusion Injury Decoy Nf-kb Circ Re Small Coronary Venule Icam-1 Dependent Pmn Adhesion Subacute Increase Intercellular Adhesion Molecule-1 Icam-1 Upregulation Inflammatory Response Early Tumor Necrosis Tnf-a Cleavage Enzyme Northern Blot Myocardial Reperfusion Venular Adhesion Electromobility Shift Assay Situ Fluorescence Microscopy |
| Content Type | Text |
| Resource Type | Article |
